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KMID : 0613820090190060787
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Journal of Life Science
2009 Volume.19 No. 6 p.787 ~ p.792
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Identification of Microorganisms Isolated from Jeju Coastal Sedimentary Layer and Characterization of Their Proteases
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Kim Man-Chul
Jang Tae-Won
Kim Ju-Sang
Han Yong-Jae
Harikrishnan Ramasamy
Han Song-Hun
Oh Duck-Chul
Heo Moon-Soo
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Abstract
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In this study, protease-producing bacteria were isolated from the marine sedimentary layer in coastal Jeju. We isolated 2 protease producing strains (SK-2 and SK-125) and tested their protesase producing activities. Gram staining and BIOLOG of isolated strains revealed that strains SK-2 and SK-125 belong to Bacillus and Pseudoalteromonas families, respectively. The 16S rDNA nucleotide sequences analyses of the isolated strains showed 99% sequence homology with those of Bacillus sp. and Pseudoalteromonas sp.; therefore, the isolated strains SK-2 and SK-125 were named Bacillus sp. SK-2 and Pseudoalteromonas sp. SK-125, respectively. The optimum conditions for the cell growth of protease activities were obtained when the both isolates were cultured at 30℃, 96 hrs and pH 7～8.
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KEYWORD
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Protease, marine sedimentary layer, Bacillus sp., Pseudoalteromonas sp.
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